This automatic cell culture system would be useful for industrialization of tissue-engineered products for regenerative medicine.
Strategies to validate advanced medicinal products have been established; however, these "best practices" still depend on the ability of personnel to perform them, such as the cultivation of stem/progenitor cells under strictly controlled conditions [ 6]. High process reproducibility can be achieved by automation, and several effective automatic cell culture systems have been reported [ 7- 12].
For this project, an automatic experiment unit (EU) was designed allowing cell culture in space.
Cells were pulsed with 18·5 kBq/well of [H]-thymidine ([H]-TdR; Perkin Elmer, Boston, MA, USA) during the last 8 h of culture, harvested onto glass filters with an automatic cell harvester (Cambridge Technology, Cambridge, MA, USA), and counted using the LKB Betaplate scintillation counter (Wallac, Gaithersburg, MD, USA).
Cells were pulsed with 1 μCi [H] thymidine during the last 8 hours of culture, harvested onto glass filters with an automatic cell harvester (Perkin Elmer, Courtaboeuf, France), and the uptake of [H] thymidine was monitored using a 1450-Microbeta Jet β-counter (Perkin Elmer).
CSF and blood samples were obtained every second hour during a 16-hours study period and were analyzed for bacterial concentrations by quantitative cultures (lower detection level: 50 CFU/ml) and WBC on an automatic cell counter (Swelab, Åstad, Sweden).
Here, we developed an automatic cell sheet stacking apparatus to fabricate 3-dimensional tissue-engineered constructs exploiting our cell sheet manipulator technique, where cell sheets harvested from temperature-responsive culture dishes are stacked into a multilayered cell sheet.
The modified PDMS was also used for cell culture.
And finally, a room for cell culture, where cells are grown for introduction to printed objects.
Furthermore, culture medium obtained from the damaged osteocytes could induce TRACP-positive cells in bone marrow cell culture.
Very recently, graphene provides a novel substrate for cell culture.
