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For British Columbia and Ontario, 200 µL and 250 µL, respectively, of sample were extracted using the NucliSENS easyMag automated extraction system and then treated with Turbo DNase ("post-DNase" treatment).
The hexane, chloroform and water extracts were prepared by using an automated extraction system while essential oil was obtained from water-distilation by using Clevenger-type apparatus.
We extracted viral nucleic acids from the swab sample by using an automated extraction system (NucliSens easyMAG; Biomeriéux, Craponne, France), and we tested the extract for respiratory viruses by using the Respiratory Virus Panel (Fast assay (Luminex Molecular Diagnostics Inc., Toronto, Ontario, Canada) in accordance with the manufacturer's instructions (4 ).
Automated extraction system provided better separation of the active principles than conventional standard extraction procedures.
The bacterial extraction from stool samples was performed manually or adapted to a fully automated extraction system.
Prairie vole BAC DNA was isolated from overnight cultures with the appropriate antibiotic using an alkaline lysis procedure or an automated extraction system (Autogen, Inc., Holliston, MA).
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A 20-μL volume of IC at a predetermined concentration was added to the sample, and DNA was extracted by manual extraction (retrospective study) or automated extraction using the EasyMag extraction system (BioMerieux, Ho Chi Minh City, Vietnam), according to manufacturer's instructions (prospective study).
For the RDT positive samples a second extraction was performed using the ABI6500 semi-automated extraction system.
Viral nucleic acids were extracted from 10 ml of chloroform-treated samples, using the NucliSENS easyMAG (BioMerieux, Marcy l'Etoile, France) semi-automated extraction system with magnetic silica, according to the manufacturer's instructions.
The GeneXpert® Dx System allows for automated extraction, processing, amplification and real-time detection of target nucleic acids.
Automated extraction also was conducted using the MagNa Pure LC extraction system (Roche).
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