Similarly, a large tandem repeat structure identified in the mitochondrial genome prevented automated closure of the remaining gap.
The total number of sequence gaps was reduced to 13 using JCVI's automated closure procedure (Hostetler et al., unpublished) which analyzes assembly results, identifies finishing targets, designs primers, selects clones, and chooses and performs sequencing reactions in an automated pipeline.
One round of automated gap closure using our in house gapResolution tool [ 46] resulted in a final assembly with 2272 contigs with an N/L50 of 139/74.8 kb.
With a combination of Sanger and second generation sequencing (that is, Illumina and 454), automated scaffolding, gap closure, error correction and annotation transfer, followed by manual inspection, we obtained highly accurate and almost complete reference genomes of PbA, PcAS and P. y. yoelii YM (PyYM).
We acknowledge the contributions by the U.S. Department of Energy Joint Genome Institute personnel to sequencing, genome closure, and automated annotation.
Here we present an example of RNA-based nanostructure design, putting emphasis on the need to characterize the structural flexibility of the building blocks to induce ring closure in the automated exploration.
In more elaborate systems, ignition, the combustion rate and closure are also automated, in addition to fuel distribution.
Plans to revamp the ticketing system to reflect that more travellers are buying tickets online, or from automated machines at stations, could lead to the closure of hundreds of booking offices.
The four main automated functions are setting train in motion, stopping train, door closure and operation in event of disruption.
Pseudo-color (red) was applied to the open, cell-free areas of each image (using an automated threshold function applied by Adobe Photoshop), in order to establish a percent wound closure measurement for each time point.
Congress needs to produce a bill that allows the Saturday shutdown as well as the closure of up to 3,700 local post offices where service would be continued through automated outlets at neighborhood businesses.
