Because full-length XB21 displayed auto-activity in the yeast two-hybrid system, an N-terminal deleted XB21 (XB21ΔNT) was expressed in yeast and used as bait to screen a rice cDNA library pool derived from leaf mRNA of 7-week old rice plants containing Xa21 (Seo et al. 2011).
Additional file 1: Auto-activity of BD-GT-4, BD-GT-4N, BD-GT-4C, AD-GT-4 and AD-TEM2 in the yeast assay.
Scaling up the auto-activation and basal activity parameters a p, o, c, b p, o, c by a factor 2.5 produces more segregated maxima in the solution, additionally, the absolute values of the copy numbers, x, y, z, are higher as depicted in Fig. 10 c.
Furthermore, Parp-1 is a structural component of the transcriptionally repressed state of chromatin, and transcription is reported to be activated by auto-modification activity in an NAD-dependent manner [ 12].
In this case, we found that phosphorylation of S184/T282 in the center domain of ZBTB16 by GSK3β may provide a signal to disrupt the inhibitory folding and thereby activate its auto-ubiquitination activity.
In addition, we show active TEVp mutants lacking auto-cleavage activity.
C-terminal WD40 repeats of Apaf-1 have auto-inhibitory activity, and either cytochrome c binding or deletion of these repeats can activate Apaf-1 (Hu et al., 1998; Riedl et al., 2005).
The coroner, however, ruled out bondage or auto-erotic activity as explanations.
Dismissing any auto-erotic activity, she said he was a "scrupulous risk assessor" and if he had locked himself into the bag would have taken a knife in with him to escape.
She dismissed speculation that Mr Williams died as a result of some kind of "auto-erotic activity", also denying there was any evidence to suggest claustrophilia - the love of enclosed spaces - was of any interest to him.
It has also been proposed that deposited vanadium sulfide may act as auto-catalytic activity for HDM and HDAs instead of being poison.
