A novel method for a large-scale preparation of active caspase-3 was developed by a strategic engineering to lack auto-activation during expression with amino acid sequences susceptible to thrombin, facilitating high-level expression in E. coli.
The precursor was strategically engineered to lack auto-activation during expression with amino acid sequences susceptible to thrombin, facilitating high-level expression in E. coli.
These served as controls to detect auto activation of bait and prey proteins.
Auto activation was more effective for documenting arrhythmias that were recorded during syncope or those with highest probability of providing a syncope diagnosis (grade 0 or I arrhythmias: auto activated, 19 patients, patient activated, 3 patients).
Of the total of 529 recordings, 223 (194 after auto activation [86.9%]) from 30 patients showed a rhythm abnormality.
Aurora B auto-activates during anaphase and activation is localized to midzone MTs [ 326].
We show here that auto-activation of Mps1 by T676 auto-phosphorylation is essential for full kinase activity and maintenance of chromosomal stability, but not for cell viability.
We confirm the existence and role of gt auto-activation through targeted mutagenesis of Gt transcription factor binding sites.
PolyP150 200 are able to support auto-activation of FXIIa and to promote thrombin generation independently of FXI activation.
Auto-activation of each transformed MaV103 strain was tested on four selective media [34].
In contrast, we observed unresponsiveness of Erk to spontaneous auto-activation of EGFR.
