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The authenticity of the selected targeted clones were checked by PstI restriction enzyme digestion and confirmed by southern hybridization by a specific probe (Fig. 1b).
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For the above-mentioned reasons, the LC-MS method was used to confirm the authenticity of two selected glycated peptides.
To investigate the authenticity of the identified SNPs, we randomly selected 50 SNPs for validation of single nucleotide variations.
To verify the authenticity of the newly identified miRNAs, we selected 13 miRNAs expressed at various levels to perform RT-PCR.
To prove the authenticity of the SOLiD, the expression levels of selected genes were validated on cultured cell lines from other cell lines of posterior UM and NUM by qRT-PCR.
Both amplicons developed the expected digestion profile, demonstrating the authenticity of the two transformants, which were then selected to be further characterized in later studies.
Interviewers were required to provide weekly updates, problems were dealt with as they were reported, and reviews at each location entailed revisiting 10% of households, randomly selected, to determine authenticity of the data.
BL21-DE3 transformants were selected on ampicillin, and authenticity of the MSP142 insert within each plasmid was verified by sequencing purified plasmid.
Questionnaires representing 10% of the location sample were randomly selected and the respective households were revisited to determine the authenticity of the data.
Female interviewers were selected to obtain a woman-to-woman interaction, which was thought to achieve higher authenticity of the information reported (15).
The Bundesbank confirmed the authenticity of the document.
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CEO of Professional Science Editing for Scientists @ prosciediting.com