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Authenticity of the cells was confirmed by DSMZ (Deutsche Sammlung für Mikroorganismen und Zellkulturen) in 2012.
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To maintain the authenticity of the cell lines, frozen stocks were prepared from initial stocks.
Experiments were conducted within a year of obtaining the cell lines to ensure the authenticity of the cell lines used.
The authenticity of the cell lines was tested by the DSMZ (German collection of microorganisms and cell cultures).
The company certifies authenticity of the cell line, and guarantees it to be free of contaminants and pathogens.
Authors should also be asked to provide hard evidence of the authenticity of the cell lines that they use as a condition for accepting a manuscript for review.
Both cytogenetic and DNA fingerprinting analyses revealed genetic identity of the three cell lines and confirmed progeny authenticity of the cell model.
To maintain authenticity of the cell lines, frozen stocks were prepared from initial stocks, and every 3 months a new frozen stock was used for the experiments.
Authenticity of the cell lines used was evaluated and verified by Bio-Synthesis, Inc. (Lewisville, TX, USA) employing genotyping of 15 short tandem repeat (STR) loci and the amelogenin gene (AMEL), and comparison with genotype information at the ATCC.
The authenticity of the cell lines was checked before starting this study, 1 year ago, through standardized techniques by evaluating the presence of a mutation in codon 13 of the Ras proto-oncogene in HCT116 cells, according to ATCC product description.
However, the authenticity of these cell lines does not compromise the conclusion and scientific significance of this paper.
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