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The drastic inhibition of tumor growth produced by uttroside B in NOD-SCID mice bearing human liver cancer xenografts, illustrates the chemotherapeutic efficacy of uttroside B, which was further authenticated by immunohistochemical analysis of the tumor sections for the expression of cleaved PARP, a classical marker of apoptosis.
The cells were authenticated by cytogenetic analysis and typing of isozymes by ATCC.
These cells were also authenticated by STR analysis.
All cell lines have been authenticated by STR analysis and are mycoplasma-free.
All cell lines have been tested for absence of mycoplasma contamination with MycoAlert (Lonza) -last control March 2013 -and authenticated by STR analysis using genRESVR MPX-2 and genRESVR MPX-3 kits (serac, Bad Homburg, Germany).
Cells have last been tested and authenticated by flow-cytometry analysis in 2009.
All cell lines were authenticated by using STR analysis (BEX, Tokyo, Japan) in July 2014.
The findings result from an extensive computer analysis, authenticated by Wolfgang Seiller, an expert on the composer.
Human cells (HEK293T and HeLa cells) were authenticated by short tandem repeat analysis (Takara).
The structure of compound 4 was also authenticated by X-ray crystallographic analysis.
GB-d1 was authenticated by short tandem repeat analysis.
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