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We next set up a similar assay permitting an authentic quantification of plasmid recombination events.
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In addition, organic synthesis of oxidation intermediates is often central to unraveling complex oxidation mechanisms and also required to provide authentic standards needed for quantification of these compounds in the atmosphere.
Furthermore, in target analysis the addition of labeled authentic standards enables absolute quantification of substances in biological samples.
We then opted for PC 23:0/23:0 because the corresponding MRM transition from human plasma (ESM Fig. S1, panel F, black trace) generates the lowest ion current (<1500 ion counts) and does not interfere with the detection and quantification of authentic PC 23:0/23:0 (red trace).
Determination of less chlorinated triclosan derivatives was not performed because of the absence of commercially available authentic standards for identification and quantification.
This approach is unlikely to have the accuracy of identification or quantification via an authentic internal reference standard due to incomplete incorporation (see methods).
Metabolites were identified through retention time and UV/visible light spectrum compared to authentic standards, followed by a total quantification with dose-response curves72.
Quantification was done using authentic sialyllactoses (Dextra) and lactose (Fluka).
Quantification was done using authentic N-Acetyl-D-mannosamine (Sigma) as external standard.
QC measurements are randomized and followed by authentic standard mixtures of 80 metabolites for establishing quantification reference points.
For LC-MS analyses, compounds were identified by retention time and MRM match to an authentic standard, integrated peak areas were used for quantification relative to external calibration curves.
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