We therefore recommend the use of Accuscript or Superscript III when dealing with low copy number RNA levels, and suggest purification of the RT reactions prior to downstream applications (eg qPCR) to augment detection.
Subcultures of Tarozzi bouillon followed after 24 h incubation at 37°C to augment detection of bacteria.
These advances, together with implementation of screening/prevention programs, have resulted in significant decline in BC mortality and augmented detection of early lesions.
Moreover, use of PCR has augmented detection of viruses that are difficult to identify with conventional methods, including human rhinovirus (HRV), human coronavirus (hCoV), human metapneumovirus (hMPV), and human bocavirus [ 7, 8].
Aside from its emerging and direct role in highly homogeneous S. enterica outbreaks, it is important to recall the important function of NGS for augmenting conventional detection, identification, and subtyping methods development.
Though their use for arsenic biosensing is in its infancy and their precision in on-site detection is yet to be established, the capability of aptamer graphene nanoensembles to augment conventional detection methods to be considered as reference points of action for risk assessments is proposed.
Therefore, detection of the Plasmodium will not only lead to proper treatment of malaria, but also augments the detection of anaemia in the same cohort.
To augment the detection of clonality in B-cell malignancies, we designed a consensus primer κ light chain gene (Igκ) polymerase chain reaction (PCR) assay in combination with a consensus primer immunoglobulin heavy chain gene (IgH) PCR assay.
For those wishing to quantify low RNA template molecules, it is advisable to augment the detection via use of a PCI-Microcon purification step, and where necessary use up to 40% of the purified RT product in downstream applications, such as qPCR.
We found that adult-onset autoimmune diabetes is prevalent in China when testing for GADA [ 1], while ZnT8A augments the detection of LADA in such Chinese patients [ 17].
