HC11 cells were maintained in growth medium (RPMI 1640 medium augmented with 10% fetal bovine serum (FBS; Invitrogen, Carlsbad, CA, USA), 5 μg/ml insulin (Sigma, Saint Louis, MO, USA) and 2 mM glutamine (Hyclone).
While the medium conditioned by Kupffer cells inhibited hepatocyte DNA synthesis, which was augmented when the medium was conditioned in the presence LPS, ALR's presence in the medium during conditioning by Kupffer cells reversed the inhibitory effect [ 22].
Finally, osteogenic potential was tested by culturing TSCs in osteogenic induction medium (Millipore Cat. # SCR028) consisting of basic growth medium augmented with 0.1 μM dexamethasone, 0.2 mM ascorbic 2-phosphate, and 10 mM glycerol 2-phosphate.
Uptake was augmented when the medium was supplemented with dibutyryl-cAMP or B27.
After 5 days, plants were transferred to new plates containing the MS basal medium augmented with phytotoxin and/or auxin treatments, and the position of the tip of each root was marked on the plate.
An overnight culture of S. aureus RN6390B was diluted 1 100 with fresh BHI medium augmented with 1% glucose, and 200 μL of that diluted culture was added to the wells of a clear 96-well microtiter plate.
Human GBM cell lines SNB19, U251 and U87 (American Type Culture Collection, Manassas, VA, USA) were grown in RPMI 1640 containing glutamate (5 mM), 10% FBS and penicillin/streptomycin. Human brain astrocytes were purchased (Sciencell Research Laboratories, Carlsbad, CA, USA) and cultured in astrocyte medium augmented with 2% FBS, 1% penicillin/streptomycin and 1% astrocyte growth supplements.
When those products do hold up their truffle flavor, it's probably because the truffle-infused medium is augmented with a chemical additive.
Right now, Blippar functions as a communications tool between brands and consumers, but the long-term vision involves making augmented reality a mainstream medium.
Expansion may be augmented using serum-containing medium supplemented with FGF-2 and incubation under hypoxic conditions [ 106, 109].
