"Until now it has been impossible to identify which of those women with atypical specimens are at high risk," said Dr. Martha Hutchinson, director of cytopathology at Women and Infants Hospitall in Providence, R.I., and one of the authors of the studies.
Some of the broomrapes growing here, which were originally thought to be oxtongue broomrape (Orobanche artemisiae-campestriae), are now no longer believed to be this species, but atypical specimens of ivy broomrape (Orobanche hederae).
When larger volumes of brain homogenate were analyzed, a characteristic <10-kDa band was present in the atypical scrapie specimen.
The amount of PrPres in the atypical scrapie specimen was so low that a maximum volume of homogenate was used.
The atypical scrapie specimen was of parietal cortex, also from a field suspect, but of the ARQ/AHQ genotype, and a corresponding negative control animal specimen was also supplied.
Non-diagnostic results encompassed unsatisfactory specimens and atypical cells when atypia was seen in the specimen, suggestive but not definitive of a benign process.
Cytological examination of the biopsy specimen revealed atypical cells forming papillary arrangement, which was suggestive of cholangiocarcinoma rather than HCC.
Fig. 3 H&E stain of vitreous specimen showing atypical epithelial cells with large and prominent nuclei arranged in small clumps (×100); Inset: TTF-1 stain of vitreous cells (×40).
The authors then became highly suspicious that she may have atypical pathogens so the specimen was also sent for acid-fast bacilli (AFB) and modified AFB stain.
