Exact(1)
Analysis of cytometry data was performed by the Attune software (Life Technologies).
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Founder Arvind Kumar says there is still plenty of room to grow in India, where Attune's software currently handles 10 million patient records for 2,500 healthcare centers, but it also plans to launch soon in the Middle East, Southeast Asia, and Africa.
Additionally, the recording level and volume were attuned so that the software could detect even the lowest sounds.
Autonomy, which was founded 16 years ago, "was based on using powerful algorithms," she said, but the software was not attuned "to new kinds of search signals, like what your friends are doing, what people like you are doing, what other data you might draw off on".
In keeping both its mobile and its desktop OS the specific province of its own devices, it can ensure that it the software and the hardware are perfectly attuned to one another.
Percentage of apoptotic cells was determined in three independent experiments by counting the number of annexin V-FITC positive cells using an Attune NxT flow cytometer and the dedicated Attune NxT software (Thermo Fisher scientific).
Using vibration-analyzing software, the team found that, instead of attuning their ears to track a male's chirrups, which range from 2.5 to 4.5 kHz, the females were listening to everything from 0.5 to 20 kHz.
After fixation with 1% paraformaldehyde, the cells were examined by an Attune Acoustic Focusing Cytometer (Applied Biosystems, Life Technologies, USA) and were analyzed using FlowJo 7.6.1 software (Tree Star, Inc., Ashland, USA).
Propidium iodide was added to a final concentration of 2 μg/ml, and cells were examined using on an Attune flow cytometer (Applied Biosystems, Zug, Switzerland) and analyzed with the Attune cytometric software v1.2.5 (Applied Biosystems).
The cells were then analyzed on the Attune Acoustic Focusing Cytometer (Applied Biosystem), and the data were analyzed using FlowJo 8.8.7 software (TOMY Digital Biology).
Cells were analyzed on an Attune flow cytometer (Applied Biosystems) and data were processed using the ModFit LT cell cycle software (Topsham, ME, USA).
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