We noted that the PKA-RIβ antibody also reacted with a second set of bands (≈35 kD) likely attributed to extraction-dependent proteolysis of the full-length expressed protein[14].
The chromatogram obtained by gas chromatography coupled with mass spectrometry showed the main compounds with different profile for nonpolar substances from Cy extract may be attributed to the extraction method.
To determine if a numerical correction factor could be used to compensate for observed discrepancies, we attempted to quantify biases attributed to DNA extraction and PCR amplification.
The absence of interference from these excipients was attributed to the extraction of CLM from the samples by methanol which does not dissolve these excipients.
The absence of interference from the excipients, even though they contain basic component(s) was attributed to the extraction of the OLM tablets prior to the analysis with methanol in which the excipients do not dissolve.
The absence of interference from the excipients, even though they contain basic component(s) was attributed to the extraction of the ATR-Ca tablets prior to the analysis with methanol in which the excipients do not dissolve.
The presence of higher concentration of guaiacyl groups in the precipitates can be attributed to the extraction of both syringyl and guaiacyl lignin groups by the lime treatments and precipitation of mostly the guaiacyl groups in the acidic conditions [44].
The increased yields of Method A may be attributed to the extraction buffer components, the appropriate concentration of Proteinase K (200 µg/mL), and optimum temperature.
The key source of systematic error in any given microarray experiment is unpredictable, but can generally be attributed to RNA extraction and, to some extent, labelling and amplification protocols.
Low recovery could be partly attributed to poor extraction efficiency (asulam, hymexazol, and, in orange, propamocarb) or degradation during sample preparation (cycloxydim, sethoxydim, profoxydim, tepraloxydim, dichlofluanide, tolylfluanide, thiodicarb, thiophanate-methyl, and, in lettuce, disulfoton and furathiocarb).
