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The physiotherapist supervising the exercise test was blinded to the attributed sequence.
The sequence assembly of GGA25 in the second chicken genome build contains 1.36 Mb of attributed sequence and the coverage is just over 2 Mb when including gaps.
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For the RNA sample preparations, sequencing libraries were generated using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB) following manufacturer's recommendations and index codes were added to attribute sequences to each sample.
Six index codes were added to each sample for attributing sequences.
Four index codes were added to attribute sequences to each sample.
following manufacturer's recommendations and index codes were added to attribute sequences to each sample.
Sequencing libraries were generated using NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (NEB, USA) according to the manufacturer's recommendations, and index codes were added to attribute sequences to each sample.
Sequencing libraries were generated using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample.
Sequencing libraries were generated using Illumina mRNA-Seq following manufacturer's instructions and ten index codes were added to attribute sequences to each animal.
Sequencing libraries were generated using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) by following manufacturer's recommendations, and index codes were added to attribute sequences to each sample.
Sequencing libraries were generated at the Novogene Bioinformatics Institute using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample.
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