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Although biological reasons such as chromosomal rearrangements between the two species could be involved, these order changes are most likely attributable to analytical causes.
The great variability described for steroid hormone receptor expression in OC is probably attributable to different analytical methods, e.g. biochemical assays like the Dextran-coated charcoal method in former times and immunohistochemistry or reverse transcription polymerase chain reaction (RT PCR) today [ 20, 24, 26, 27].
Thus, we cannot rule out possible variability over time attributable to the different analytical methods used and the fine-tuning and improvement of the related quality-control measures.
It is therefore very important to develop analytical methods in order to find the solutions.
These deep structural similarities can be exploited to transfer analytical methods and understanding from one field to another.
In our study, careful consideration was given to standardization of analytical methods and instrumentation.
An approach that may yield successful results is to use different analytical methods to select individuals according to phenotypical characteristics.
This is an alternative method to classical analytical methods.
Our results demonstrated that the GTI provided valuable complementary information when compared to existing analytical methods.
Immunofixation performs comparably to other analytical methods such as isoelectric focusing [31].
Such difference could be attributable to the methods used for monitoring cGMP in both works.
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