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PCNA functions in cell cycle progression and serves as an attractive assay of cell proliferation; Ki-67 is a nuclear protein that is expressed in proliferating cells and may be required for maintaining cell proliferation.
Although its simplicity makes the MLR an attractive assay with which to examine in vivo responses, the overall clinical experience has been that mixed lymphocyte responses do not provide a reliable prediction or reflection of graft outcome 28 and, indeed, we have previously noted a clear disparity between the in vivo and in vitro suppressive capacity of CD25+CD4+ Treg 29.
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A particularly attractive SNP assay has been the colorimetric sensing using Au NPs because of their extremely strong surface plasmon resonance (SPR) absorption at ∼520 nm (ε > 10 M–1 cm–1 for a 20 nm Au NP, 4 5 orders of magnitudes stronger than typical organic dyes), making it strongly colored even at low nM concentrations.
Taken together, imaging of αvβ3 integrin expression is an attractive tool to assay the post-MI angiogenesis and may be potentially used to predict outcome of infarct healing.
Additionally, the use of banked cryopreserved PBMC samples makes this assay attractive in the setting of large efficacy trials where it is less feasible to perform immunoassays on freshly isolated samples.
However, as noted above, whether or not NH4Ac is attractive depends on assay method.
Therefore, specificity, sensitivity, speed and real-time detection make these assays attractive for use in epidemiological and ecological studies.
MDI queens elicited a stronger retinue than SDI queens in natural colony conditions, and their mandibular gland extracts were more attractive in preference assays with caged worker bees.
However, for smaller scale projects where potential genes are already known, technologies for genotyping of many samples instead of SNPs and in addition retain high accuracy and throughput, are more attractive compared to assays that are cost effective per SNP.
Administration of deuterium oxide and collection of skin samples is very easy making this assay highly attractive; however as with any new assay feasibility and cost must be considered.
Results can be available within 3 hours of receiving a broth sample, which makes this rapid and simple assay an attractive diagnostic tool for clinical use.
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