Cell migration was observed in culture media containing 2% FBS to attenuate cell motility.
The results showed that the PI3K inhibitor LY294002 markedly attenuated cell motility (3-fold) of two clones overexpressing wild-type ezrin as well as cells transfected with empty pCB6 vector (data not shown; Fig. 4a,b).
In vitro analysis indicated that bexarotene attenuated tumour cell motility and inhibited cell invasion through reconstituted ECM.
Inhibition of effector kinases by the PI3K/mTOR inhibitor, PF1502, decreased C-terminal phosphorylation of p27, reduced its cytoplasmic localization, and attenuated tumor cell motility, invasion and metastasis.
Extracellular zinc also attenuated cell death.
As shown in Fig. 5B, down-regulation of Rac1 or IQGAP1 with siRNA (50 nM, 48 h), compared to scrambled siRNA (50 nM, 48 h), attenuated [S1P]ext-induced cell motility.
Erlotinib not only effectively abolished the motility of PC-9 cells but also attenuated the enhanced cell motility of PC-9ER cells (Fig. 1a,b).
In contrast, C3 did not attenuate bFGF-induced cell motility, indicating that Rho, unlike Rac, is dispensable for the bFGF-induced motility of ESFT cells.
The CCR5 and AMP-activated protein kinase (AMPK) inhibitor or siRNA also attenuated CCL3-upregulated cell motility and MMP-2 expression.
Conversely, the LOX pro-peptide (LOX-PP), which is released during the maturation of the pro-LOX enzyme, reduces breast cancer cell motility by attenuating phosphorylation and activation of p130Cas/BCAR1 [ 20].
