Comparisons of single and combined stress effects on growth and biomass traits exhibit patterns of allele effects that suggest attenuating interactions among physiological signaling steps in drought and ultraviolet radiation stress responses.
The presence of attenuating interactions implies that shared QTL found in sets of environments could be used to group environment types and identify underlying environmental similarities, and that patterns of stress-dependent genetic architecture should be studied as a way to prioritize prebreeding populations.
Engineered mutations within this hydrophobic patch attenuate the interaction with the Mlp1 C-terminal domain but do not alter the interaction with Gfd1, indicating that this patch forms a crucial component of the interface between Nab2 and Mlp1.
Deleting Robo1's Ig1 domain does not significantly attenuate the interaction with Robo2, but further experiments will be necessary to determine if Robo2 interferes with Robo1's interaction with Slit.
Overall, while residual confounding due to these or other factors might be expected to further attenuate the interaction effect, the above comments and the extent of the attenuation of the breastfeeding effect due to the 7 factors used in this study may suggest that the main sources of confounding have been accounted for.
Adjusting for confounders did not fully attenuate the interaction between gender and neighbourhood socioeconomic circumstances (table 2, model 3).
Further co-IP experiments proved that overexpression of KSR1 attenuates the interaction of SIRT1 DBC1, which therefore allows SIRT1 to interact more with p53 resulting in decreased p53 acetylation.
Even though the minimal WD40 domain coIPs only weakly with ChiLS, the same is true for full-length Groucho, while Δlinker interacts very strongly, suggesting that the linker attenuates the interaction between Groucho/Tranducin-like enhancer protein (TLE) and ChiLS, consistent with the known regulatory role of this region (controlled by phosphorylation; Turki-Judeh and Courey, 2012).
De-conjugation of SUMO2/3 by SENP3 attenuated the interaction of Sp1 with RNF4.
Both chemicals attenuated the interaction between p50 and Annexin A2-WT, which was either endogenous or myc-tagged exogenous.
