Exact(6)
We nevertheless attempted to complement a pfa4Δ strain using Swf1 constructs bearing the single point mutations, the triple mutant and a construct with two additional SDPs mutated to the corresponding residues in Pfa4, and neither complemented (not shown).
However, whenever we attempted to complement the knockouts we would discover that differential expression of motility genes was not affected by complementation.
To confirm that the lack of production was due solely to the deletion of cxmK, we attempted to complement the mutant by expression in trans of a wild-type copy of cxmK cloned in the expression vector pIJ10257.
To assess functionality of the SLEEPER genes found in other species, we attempted to complement the daysleeper phenotype with coding sequences from rice and grapevine under control of the 3.6 kb upstream region of DAYSLEEPER, including the 5'UTR.
Moreover, we attempted to complement our logfile analyses with an online survey but the low response rates, even when we offered an incentive to respond, highlights the challenge of gaining insights from a group of busy doctors, something that needs to be improved to enhance ongoing monitoring activities.
We attempted to complement the RNAi approach with re-expression of a siRNA-insensitive DNMT1 RNA, but pilot transient expression experiments using lipofectamine and an empty vector (pcDNA, Invitrogen) failed, likely due to the inability of THP-1 macrophage to survive multiple transfections during differentiation.
Similar(54)
Warner attempts to complement the songs with snatches of video projection, combined with different backdrops, petals raining from the flies and a mimed sex scene under the piano.
This paper attempts to complement the scientific papers in this special issue of Ecological Processes.
We also examined PHB content in Re2005 (ΔphaZ1 ΔphaZ2 ΔphaZ3 ΔphaZ5) cells containing plasmids pER1-4 under PHB utilization conditions (Figure 2), attempting to complement the PHB utilization phenotype of the strain.
Online car-buying startups are increasingly attempting to complement and supplant the existing brick-and-mortar sales model, too, and even Amazon has experimented with online sales, particularly in Europe.
The innovation assets begin as fundamental science and engineering, patents and business plans, potentially turning into new products or even entire lines of business for "intrapreneurs" attempting to complement or extend the company's core business.
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