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While this was a statistically significant increase in yield, it was far below the millions per gram reported for other species [ 22], it did not work consistently between isolation attempts, and was not a large enough improvement to attempt culture and fusion.
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Herein, we focus on characterizing the S. amnii isolate, which we have successfully cultured and cloned, although we are continuing to attempt to culture and clone vaginal isolates of S. sanguinegens and the third, as yet unnamed, putative Sneathia.
There's almost a satiric urge in it, I think, a kind of curdled attempt at culture jamming.
We did not attempt to culture the Trichococcus spp, as there was only sufficient sample to do a gDNA extraction for 16S rRNA PCR but not for culture work.
Taken together, the factors cited above provide a strong rationale to attempt OMS culture in the Spermondes.
Three methods were used concurrently to attempt to culture B. pseudomallei from water at each sampling site.
As far as we know, the only attempt to culture PGC-LCs in vitro within embryo-derived ovarian tissue has been reported by Hayashi and Surani.
We also attempted to culture other bacteria and fungi species, but not all were successfully cultured (data not shown) since some species were slow-growing or difficult to culture, and the samples were stored at −80 °C before use.
The Waikiki aquarium is attempting to culture the new corals and present them in an 2011 exhibit dedicated to the monument.
This effort resulted in the complete, or nearly complete, genomes for a handful of bacteria and archaea that had previously resisted attempts to culture them.
Previous attempts to culture MCs were performed in total epidermal cell cultures in which keratinocytes were predominant.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com