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For each combination of paired genomes, rather than attempt alignment, we used the portable implementation of tblastx [29] available in the NCBI Blast distribution (http://www.ncbi.nlm.nih.gov/blast/download.shtml).shtml
One method used by the BWA alignment process to establish the correct location of an ambiguously mapping read is to attempt alignment within a limited region defined by the mapping location of the other read in the mate pair [ 31].
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Due to the relatively low degree of sequence conservation at the nucleotide level within some of these families, phylogenetic inference was carried out on amino acid alignment, rather than attempting alignment at the nucleotide level.
The distal femoral condyles were resected to attempt an alignment of 6° valgus in the coronal plane.
Global alignments attempt to align entire sequences, up to both ends of each sequence [ 1].
Closer inspection of the corresponding sequences, and manual attempts at alignment, were subsequently able to demonstrate that all of the unidentified ESTs were also myoglobin, including additional examples of a unique brain-specific isoform [ 11].
If C[i,j] is the query gap, then Barnacle attempts an alignment between C[i,j] and C[0,i-1] + C[j + 1,|C|-1] (i.e. the concatenation of the sequence before and after the query gap).
An attempted re-alignment of some secret wrongness in the author's life?
These were also surrounded with packs of sausages and re-placed on the motion table as in a patient acquisition procedure, with no attempt to keep alignment orthogonal by any other means than manual placement.
Sequences from the non-coding regions (trnH-psbA, atpF atpH, and psbK psbI) were usually only readily alignable within genera, and so we did not attempt higher-order alignments for them.
Structural alignment attempts to establish homology between two or more polymer structures based on their shape and three-dimensional conformation.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com