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This growth-flexibility trade-off gives a straightforward interpretation to recent work showing that most overall cell-to-cell flux variability in a population of E. coli can be attained sampling a small number of enzymes most likely to constrain cell growth.
The attained sampling fractions for CIN1 and negative biopsies were smaller than targeted because of lack of residual tissue (Supplemental Tables 1 and 2, Supplemental Digital Content 1, http://links.lww.com/PAS/A253).lww.com/PAS/A253
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The attained sample at nine months was 18 552 children and families (response rate 70%).
Stable chemostat cultivations were attained within two or three residence times, and three generations after steady states were attained, samples were withdrawn for transcriptomic and proteomic analyses.
While every effort was made to include as many returnees as possible in our study, the attained sample size is relatively small.
With the attained sample size in these three groups, we achieved 90% power to detect a difference of 0.75 on the EURO D scale given the standard deviation in our sample of 2.8.
With the attained sample size in each group, there was a >95% power to detect a 1-SD change in BMI, FPG, 2-h glucose, or A1C levels between the study groups.
For a two stage design, current practice often relies on ignoring the over- or underaccrual or in re-computing the decision boundaries as if the attained sample size had been planned in a single-stage design, which leads to bias and possible inflation of the type I error rate.
To attain sample power the whole school year was surveyed in seven schools which had relatively small year groups.
In addition, we terminated the sampling process when we attained a sample size sufficient to test our primary hypothesis, without maximizing response rate.
However, and in many situations, maximum precision is attained when sampling consists of replicates of specific experimental points.
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