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After confluence was attained, cells were subcultivated by a 5-min incubation in 0.1% trypsin and 5 mM ethylenediaminetetraacetic acid (EDTA).
When these objectives cannot be attained, cells generally undergo RCD as a means to preserve the homeostasis of the whole organism (or colony, in the case of yeast cells).
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It remains of vital importance to identify and characterise cells responsible for metastatic relapse at the molecular level, with the potential reward of identifying strategies to target therapy towards the prolongation of dormancy or to attain cell death (Epstein, 2005).
One of the main TORC1-regulated roles of Sch9 is to modulate translation and cell size attained before cell division (Jorgensen et al., 2002, 2004).
In asynchronous growth, T36E cells attained a larger cell size before exiting G1 compared to SSA1 or T36A cells, suggesting a role for Ssa phosphorylation in delaying S phase onset.
Best production performance was attained when cells were transfected at mid-log phase (2 3 × 106 cells/mL) with medium exchange at the time of transfection using standard amounts of plasmid DNA and polyethylenimine.
Each inoculation of 3 L culture attained 109 cells and could be induced to continuously express VLPs three times.
Embryos that have not attained 16-32 cells and that did not exhibit compaction were classified as early degenerate.
The adaptive alterations attained by cells to withstand different types of insults are collectively referred to as the stress phenotypes of cancers.
In contrast to our approach, this state was not attained in cells transduced with wt hPIV3 HN or the NA-deficient mutant HN (C28a), since homologous superinfection was detectable [ 10].
Stable pools and cell lines expressing E2F-1 were isolated that attained viable cell densities 20% higher than control cell lines and continued proliferation for an additional day in batch culture.
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