Many columns with fused core technology were also evaluated to attain resolution between LS and LHA, but due to high back pressure, these columns could not be used.
Using the specially designed photoelectron photoion apparatus, which features a supersonic molecular beam source and opposing electron and ion time-of-flight spectrometers, we have recently succeeded in performing synchrotron-based PFI-PE photoion coincidence experiments, attaining resolutions limited only by the PFI-PE measurements.
If your computer can't attain such resolutions, it's possible to work around the limitation with special software, but that's venturing into professional territory.
However, in-focus imaging with VPP requires very precise focusing7 and the typically strong Cs present in cryo-electron microscopes appears to be a limiting factor in attaining resolutions better than 3 Å by in-focus phase plate imaging7.
This method can attain higher resolution by implementing electrodes in or near boreholes and monitoring the electric potential distribution near the horizontal fracture zone.
STED and PALM technologies can attain better resolution, 50 and 20 nm, respectively (Rust et al. 2006; Shim et al. 2012; Jans et al. 2013).
Thus, although the combination of spectratyping with sequencing can attain sufficient resolution to analyze TCR diversity, the approach is laborious and time consuming as it requires PCR amplification, isolation of individual bands based on DNA size, purification, followed by repeated cloning and sequencing.
Modifications made to the B. subtilis SSB probe allow us to attain high resolution ssDNA binding signals in complex multi-protein assays with high tryptophan backgrounds.
In summary, US is inexpensive and convenient and can be performed in real-time, whereas MRI can attain high-resolution images and provide thermometry data.
The number of cases of functional dyspepsia responsive to treatment is low [ 34], as only 50% of ulcer patients attain symptom resolution [ 35, 36], whereas the symptoms of patients with reflux disease do not improve with treatment [ 37].
