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In cell attachment test, MSCs and chondrocytes adhesion to synthetic polymers in 1 h were very low and ranged from 2.8%to8.0%0%.
The effects of each polymer fiber on cell adhesivity, proliferation, morphological changes, and synthesis of the extracellular matrix were analyzed by quantitative a cell attachment test, DNA quantification, light and scanning electron microscopy, semi-quantitative RT-PCR, and immunohistochemical analysis.
The cell attachment and proliferation were significantly higher on PLGA/HA (10 wt.%) composite film than control group (1.44 times higher in attachment test and 1.31 times higher for the 6th day at culture in proliferation assaying, p < 0.05).
However, the surface of the 4-hexylresorcinol-incorporated 4-hexylresorcinol-incorporated 4-hexylresorcinol-incorporatedellular attachment test.
The recent study by Kwon et al. [27] evaluated in vitro (cell attachment test) and in vivo (implantation test on the rabbit tibia) biocompatibility of solid freeform fabrication (SFF) and polymer replication method (PRM) HA scaffolds with 45%% porosity.
Spiroplasma invasion of Ciha-1 cells was estimated similarly to the attachment test after a gentamicin protection assay.
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Bacterial attachment tests proved that membranes could also resist micro-biofouling.
Furthermore, these composite membranes are biocompatible as evident by neuronal cell attachment tests with in vitro PC12 cell lines.
And then, cell-attachment tests were performed as described above.
Thus, the result of this attachment-test was 100% consistent with the results of infection trials (Table 1).
However, the binary polymorphism found in infection trials with high doses of single parasite clone [ 20] correlates perfectly with the results of our attachment-test (Table 1).
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