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Arguably surprisingly, a conserved phenylalanine at position 15 in the yeast protein and 11 in horse heart, but in each case separated by only three residues from the CXXCH motif, and for many years thought only to be essential for cytochrome c structural integrity, has been shown to be essential for heme attachment but not necessary for properly folded protein.
Similar to WT DHCR24 V5, both truncations were found primarily in the membrane fraction, demonstrating that the first 240 residues are also not necessary for membrane attachment.
Interestingly, the attachment of telomeres to the NM is not necessary for telomere integrity or function, since in a bqt4Δ mutant background, telomeres are no longer bound to the NM without any obvious consequences for telomere length or silencing.
Using a Flex-Shaft Attachment will make this easier, but it is not necessary.
It was not necessary.
"Not necessary," Archer says.
Experience is not necessary.
Reservations not necessary.
Reservations are not necessary.
It's not necessary".
Actual words not necessary.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com