The microarrays were blocked by attachment upside down to a 60 ml drop of 3x SSC, 0.3% N-lauroylsarcosine, 60 mM Tris-HCl pH8.0, 83 ng/ml denatured herring sperm DNA and 1% BSA over a Hybri-Slip coverslip (Sigma) and incubated at 42°C in a water bath for 30 min.
To determine when the whipping cream has reached the soft peak stage, turn the whisk or mixer attachment upside down, breaking the surface of the cream.
The auditorium is a giant raw cube of a space, perhaps 50 feet on a side, the ceiling stripped down to rough red and umber terra cotta blocks, with old metal ceiling attachments hanging down like flypaper strips.
The basis of attachment ultimately boils down to an emotional connection to things, places, ideas, and more.
Extensive damage was observed to occur in the hold-down attachment and in part of the bottom track adjacent to the hold-down, which prevented the wall panels to exhibit the stiffness associated with the main load resisting mechanisms.
Smaller surgical tools with ultrasound attachments that break down the fat before it is suctioned away have also made the procedure safer.
Experimental results revealed that the geometry of hold-down attachment used at the base of CFS wall panels to transfer tensile forces has a major effect on the overall behavior of the panels.
Psychologists say it comes down to attachment theory: that catastrophes, in short, have a way of driving people together and influencing major life decisions.
All children, even those with loving parents, even those with attachment parents, will fall down occasionally, feel sad, be insecure, get angry, and that's not because they had bad parents – it's because they're human.
For previous series, puppet eyes had been sized out of proportion to the heads, but as part of the realistic look introduced in Captain Scarlet, the eyes of production personnel were photographed and the images scaled down for attachment to the eye sockets.
