The formation of a stable bipolar meiotic spindle is crucial for chromosome disjunction in which chromosome attachment, alignment and pulling toward spindle poles are critical steps for accurate segregation.
Finally, endothelial cells are highly dependent on tubulin cytoskeleton for their motility, invasion, attachment, alignment and proliferation (Denekamp, 1982).
RGD surface modification enhanced cell attachment, proliferation, alignment and expression of both collagens (type I and V) and proteoglycans (decorin and biglycan).
Confocal images further confirmed normal cell attachment and alignment of actin filaments within the porous scaffold matrix with well-developed nuclei.
Our results indicate that MNF+PIEZO scaffold promotes rat and human cardiac cell attachment and alignment, maintains the ratio of cell populations overtime, promotes cell-cell communication and metabolic maturation, and preserves cardiomyocyte (CM) contractility for at least 12 days.
Highly Expressed in Cancer protein 1 (Hec1) is a constituent of the Ndc80 complex, a kinetochore component that has been shown to have a fundamental role in stable kinetochore-microtubule attachment, chromosome alignment and spindle checkpoint activation at mitosis.
For example, several studies have shown that single-digit micrometer and sub-micrometer surface features can be used to control cell attachment and alignment [ 19, 59– 61].
The directionality of cell movement is maintained by Cdc42, which coordinates actin polymerisation at the front of the cell with microtubule attachment and alignment [ 57, 58].
Besides, its middle domains contain two heterodimers of Ndc80-NUF2 Spc24-Spc25pc25, whave have been suggested to be necessary in formation of stable microtubule kinetochore attachment, chromosome alignment, and spindle checkpoint activation in mitosis [ 10].
This indicates misaligned chromosomes in GAK-depleted cells have either failed to attach to microtubules or are unable to maintain microtubule attachments, but alignment defects are unlikely due to a failure to release incorrectly attached microtubules.
BubR1 is hyperphosphorylated during mitosis, and phosphorylation of the BubR1 KARD domain by Cdk1 and Plk1 has been proposed to play a key regulatory role for BubR1 in establishing error-free kinetochore-microtubule attachments and alignment of duplicated chromosomes at the metaphase plate.
