Exact(1)
His group developed a binding assay by attaching purified β-catenin, including the TCF binding site, onto a plate (Fig. 2).
Similar(59)
The structure of the N-linked glycans attached to 5 μg of purified HCG were determined according to the method of Royle et al [ 9].
A successful strategy for immobilization of membrane proteins is to attach the purified protein, by using the selective affinity of a genetically introduced histidine tag (His-tag), to a nickel-chelating nitrilotriacetic acid (Ni-NTA) monolayer (SAM) self-assembled on a chemically modified gold surface.
We analyzed the structures of N-glycans attached to PSA purified from sera of patients with prostate cancers and of those with benign prostatic hyperplasia (BPH) [ 20].
The material was thoroughly purified from attached, non-tracheal material.
The attached templates were further purified with 0.5× and 0.45× bead-washes for sequencing.
The final product DS-SILY n, where n indicates the number of attached SILY peptides, was purified in ultrapure H2O, lyophilized and stored at −20 °C until use.
Dye-labeled heterodimeric kinesins (0.5 2 nM) were attached onto the axonemes (purified from sea urchin sperm flagella) in the presence of 1 mM AMP-PNP, or moved along the axonemes in the presence of ATP and an ATP-regenerating system as described (Mori et al, 2007).
For negative staining, purified VLPs were attached onto a carbon-coated grid for 5 min at room temperature.
Current vaccines activate T cells and thus the CD40 receptor by using purified bacterial proteins attached to a number of different polysaccharides.
BALB/c mice were immunized by intrasplenic deposition of 1 μg of the purified fusion protein attached to PVDF membrane for the first time [ 22].
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