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The effects of this coating agent on the transfection of loosely attaching cell lines were studied.
Collectively, these results suggest that in an attaching cell the cytoskeletal network in the apical domain might restrict membrane-protein diffusion throughout the cell and provide a simple mechanism for a local signaling centre to facilitate the formation of a polarized cell.
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Attaching cells were cultured for two weeks under standard conditions.
Antifouling surfaces using a protein mimetic polymer were also prepared for attaching cells [5].
Another interesting novel approach is to deliver purified proteins to the cells by attaching cell-penetrating peptides to the purified protein constructs or RNA:protein complexes (in the case of CRISPR/Cas9), therefore allowing spontaneous uptake by the cells [ 132- 134].
At this time the human mesenchymal cells had attached to all surfaces and were rounded or spindle shaped, which is typical for early attaching cells.
In particular, during influenza infection, the virus attaches cell surface receptors containing sialic acids.
Evaluation of cell adhesion was conducted by counting stained nuclei of attached cell per square centimeter of the substrate surface.
The attached cell number and type I collagen formation on TCP/HA were more than that on HA.
This orientation means that the centrosome in the attached cell is oriented towards the substrate and to the side.
Apoptosis was examined in the floating and attached cell populations.
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CEO of Professional Science Editing for Scientists @ prosciediting.com