The phrase "attached was removed" is not correct in standard written English.
It seems to be an incomplete thought and lacks clarity regarding what was attached and removed.
Example: "In the email, I noticed that the document attached was removed before sending."
Alternatives: "the attachment was deleted" or "the file was taken out".
For the thawing, the film with the CPA loaded RBC droplets attached was removed from the liquid nitrogen using tweezers and immediately transferred in a petri dish filled with a thawing solution consisting of 10 ml of 2.5M glycerol pre-warmed to 25°C (Supp. Fig. S1c).
To isolate pre-apolysis sensory epithelium from pupae (pupation through about 72 hrs a.p ., the outward facing antennal pupal cuticle with epithelium attached was removed using a scalpel and immediately fixed in 4% paraformaldehyde (PFA) in PBS-Tx 0.3%) PBS-Tx 0.3phosphate, 150 mM NaCl, pH 7.0, containing Triton X at 0.3%) and processed as described below.
A dorsal section of the abdominal wall with adhering fat body tissue (the major site of vitellogenin gene activity in honey bees [41]) attached was removed and frozen in Trizol reagent for later RNA extraction and vitellogenin mRNA quantification (see below).
The heart, with thoracic aorta attached, was removed and placed into a physiological salt solution of composition (mM): NaCl 154, KCl 5.6, MgSO4·7H2O 1.2, HEPES 10.9, glucose 8, and CaCl2 2 (adjusted to pH 7.4).
The solidified agar drops with zoospores attached were removed at various time intervals.
The cell/bead suspension was placed on a strong magnet and the ferrous beads with cells attached were removed by magnetic adhesion.
The CD31-negative cells (with no beads attached) were removed during the successive washings.
The brains with the olfactory organs attached were removed and postfixed in the same fixative for 24 48 h at 4 °C.
Aphid heads with the intact digestive tract attached were removed by applying light pressure anteriorly with the pin and posteriorly with the forceps.
Further, for each individual experiment, depending on the used annotation database, genes without any annotation attached were removed from both sets.
Eyes with optic nerve segments attached were removed from the connective tissue, post-fixed for several hours in a 4% PFA, transferred to 30% sucrose overnight (4 °C) and embedded in Tissue-Tek (Sakura, Alphen aan den Rijn, Netherlands).
