The BG11 medium was dripped onto the filter paper with a speed of 0.5 mL min−1 to keep the algal disks attached to the filter paper and provide nutrients for the growth of the algal cells.
Invasion was measured after 6 hours after which time gels were removed with a cotton swab, the filter washed in PBS and stained with calcein (Invitrogen) and the number of cells attached to the filter was counted.
The endotracheal tube was attached to a filter (Hygrobac), and both were set to ASL 5000 active servo lung (IngMar Medical).
All further manipulations, including particle-mediated acute gene transfer, were carried out with the retina attached to the filter.
The pieces of retina still attached to the filter were embedded in Tissue Tek in an aluminum foil boat and frozen in dry ice.
The biotinylated PCR products, attached to the filter probes of the vacuum tool, were immersed in 70% (v/v) ethanol for 5 seconds, denatured in PyroMark Denaturation solution (Biotage AB 40 0034) for 5 sec (allowing only the biotin labelled strand of the PCR product to stay attached to the filter probes) and immersed in 1X PyroMark Wash buffer (Biotage AB 40 0035) for 5 sec.
For the in vivo microscopy of the transplanted endometrial tissue fragments, the anesthetized mice were fixed on a Plexiglas stage and the observation window of the dorsal skinfold chamber was positioned under a Zeiss Axiotech microscope (Zeiss, Oberkochen, Germany) equipped with a 100-W mercury lamp attached to a filter block for blue, green and ultraviolet light.
Cells pretreated with NS-398 or vehicle were loaded into the upper chamber, and, following a 24-h incubation period, the number of cells attached to the filters below the Matrigel layer was counted.
The show also includes other interesting but perhaps less successful conceptual projects, like Stacy Levy's cleverly made installation of hundreds of pieces of nylon thread tufts attached to a window to filter and reflect the soft afternoon sunlight.
This type of attack has grown in popularity, in part because executable files (e.g., *.EXE) attached to emails are filtered by most email servers due to the risk they pose and also because non-executables (e.g., *.PDF, *.DOC, etc).
