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The repeated tag sequence is attached to a sequence end part, e.g., 3'-untranslated region (UTR) of the target RNA, which has a minor influence on the RNA structures and functions.
Sequences were clustered with their primers attached to a sequence identity threshold of 0.8 and assigned to clusters by the highest identity across all clusters.
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The 467 bp orf265 sequence was then attached to a neighboring sequence, generating the nap form of orf261.
RNA modules are intrinsic to RNA architecture are therefore disconnected from a biological function specifically attached to a RNA sequence.
The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode.
AGP has five N-linked chains (so called because they are attached to the side chain of an asparagine amino acid) which can have a variable number of branches attached to a core sequence.
We found that the sequences CGCC and GCC were often attached to a TATA-box sequence upstream of Fe deficiency-upregulated genes.
Additionally, all reverse primers were attached to a PIG-tail sequence (5′-GTTTCTT-3′) at the 5′-end of the sequence (Brownstein et al., 1996).
Three different synthetic ORF's dubbed VDI, VDII and VDIII, each encoding a trimmer of the vessel dilator peptide attached to a His tag sequence at their C- terminal, were synthesized and placed in pET21c expression vectors.
The 200-bp reference DNA (the 191-bp bacteriophage fd gene VIII, sites 1350~1540 attached to a 9-bp sequence, CTACGTCTC, at the 3'-end) is experimentally determined to have a melting temperature of 60°C under standard conditions.
In brief, double-stranded cDNA was synthesized from 10 μg total RNA using the SuperScript Choice System from InVitrogen and the Affymetrix T7- dT 24 primer which contains a T7 RNA polymerase promoter attached to a poly-dT sequence.
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