The attached signal tags can be easily read out by SERS.
This covalently attached signal is assembled from multiple ubiquitins linked to each other via the C-terminus of one ubiquitin and the ɛ-amine of Lys48 of another ubiquitin.
Ubiquitin has originally been characterized as a covalently attached signal for ATP-dependent proteasomal degradation of substrate proteins (Hershko and Ciechanover, 1998), although it also plays a role in both the lysosomal and autophagic degradation pathways (Clague and Urbe, 2010).
The closest annotated gene to the 5p15.1 macrosatellite repeat is BASP1 (Brain Abundant, membrane attached Signal Protein) located approximately 210 kb distal to the array.
For example, we found that brain expressed genes BASP1 (brain abundant, membrane attached signal protein 1), BEX1 (brain expressed X-linked 1) and BEX2 (brain expressed X-linked 2) were up regulated after SOX2 knockdown (Table 4).
They include EBF3 (early B-cell factor 3), BASP1 (brain abundant, membrane attached signal protein 1), SLC30A1 (solute carrier family 30 (zinc transporter), member 1), and SLC3A2 (solute carrier family 3, member 2).
In a more recent study, Tang et al compared the NP and AF gene expression and again identified CD24, but also brain abundant membrane attached signal protein (BASP1), N-Cadherin (N-Cad), neuropilin (NRP-1), CD155 and CD221 as specific rat NP markers.
We have previously shown that zinc on the surface of the QD can bind histidine-tagged peptides and proteins such as maltose-binding protein (Boeneman et al., 2013), and that by using the QD as a delivery vehicle, we can attach signaling peptides to the surface to further specify the target location.
In particular, junctions with fluorescein attached gave signals that allowed the measurement of RepD binding kinetics.
In this study, we designed transgenes of annexin V with or without an attached secretory signal peptide and investigated the secretion of the transgene products in COS-7 cells.
At 6 am on 28 May the attached frigate signalled Bellerophon to report a sighting of a strange fleet.
