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Nevertheless, the study found MARs to be slightly enriched in gene poor regions and relatively absent from transcriptional promoters, and SARs were also enriched in gene poor regions, whereas skeleton-attached sequences were associated with gene rich regions and CpG islands.
RGD-based peptide sequences were attached to promote endothelial cell adhesion, because vascular grafts seeded with endothelial cells have fewer problems with thrombosis.
Alternatively, peptide sequences were attached via a chemoselective reaction, which involved the addition of the sulfhydryl group of a cysteine residue of an oligopeptide to a maleimide moiety present on the surface of the dendrimer.
Codon-optimized versions for prokaryotic expression of AviTag™ (N-MSGLNDIFEAQKIEWHE-C), FLAG-tag (N-MDYKDDDDK-C) and the HIS6-tag (N-MHHHHHH-C) peptide sequences were attached 5'-terminally to the pVII ORF in M13K07 by QuikChange™ in vitro mutagenesis according to the manufacturers' protocol (Stratagen, LaJolla, CA, USA).
The ends were polished and the 454 titananium adapters containing specific MID sequences were attached.
The primer sequences were attached in Additional file 1: Table S2.
Specific 10-bases long barcode sequences were attached to primers as a tag for identification.
Additional 37 base pair of universal tag sequences were attached to each forward and reverse primers.
M-13 phage forward standard primers sequences were attached to the 5′ of the core primers to decrease primers dimer formation.
IKVAV peptide sequences were covalently attached to an aminated polymer surface using carbodiimide chemistry.
To enable subsequent identification of metastasis-promoting DNA sequences, the fragmented genomic DNA sequences were covalently attached to specifically engineered linker DNA molecules to flank the genomic DNA before transfection.
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