For scanning electron microscopy, the sample was allowed to settle on the surface of a poly- l-lysine-coated glass cover slip for 30 min, and the attached sample was then fixed with 0.1% glutaraldehyde in 0.1 M phosphate buffer (pH 7.4) for 10 min, washed three times with 0.1 M phosphate buffer, and post-fixed with 1% osmium tetroxide in the same buffer for 10 min.
For sperm acrosomal integrity analysis, sperm were stained with LysoTracker DND-26 (Invitrogen, Carlsbad, CA, USA) at RT for 30 min. For ADAM3 immunocytochemistry, sperm were attached to a glass slide by cytospin centrifugation, and the attached sperm samples were fixed with methanol at RT for 5 min and immunostained with anti-ADAM3 antibody.
However, contact-mode imaging turned out to be less suitable for weakly attached samples, because bio-molecules are often pushed away by the AFM stylus during imaging (9).
"Ninety percent of our production is exported," said Ian McCormick, international managing director for Equalizer, standing beside a yellow mock-up pipeline to which were attached samples of his company's stainless steel clamps.
After a piece of duct tape was found with strands of blond hair attached to it, samples were tested both at a Dutch lab and at Quantico.
A monochromatic x-ray beam tunable to energies from 2.0 to 17 keV using a Si(111) crystal set is focused on the sample using a Kirkpatrick-Baez mirror pair.[21] The shale samples were attached to the sample holder using double-sided tape and the sample holder is attached to an XY stage that is oriented 45° to the beam.
The punched samples were attached to gold wires using silver paint (DuPont 4929N).
The samples were attached by cotton thread to sheets of polyester (Melinex™) and laid horizontally.
Samples were attached on the top of an aluminum stopper by means of carbon conductive adhesive tape.
The aminosilane-modified PS samples were attached to DMSA by wet impregnation followed by the adsorption of As (III).
