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However, all these treatments resulted in no detectable L. monocytogenes cells upon their application against the mixed-culture sessile communities also containing the fish indigenous microflora, something probably associated with the low attached population level of these pathogenic cells before disinfection (< 102 CFunder) under such mixed-culture conditions.
The attached population was also examined for the formation of giant, polyploid cells.
In all cell cultures there was no evidence of an increase in giant cells in the attached population.
The attached cell population was also analysed for the presence of apoptotic cells by acridine orange staining; in all cell cultures less than 1% of the attached population showed morphological features of apoptosis.
An increase in morphological features of apoptosis or an increase in giant polyploid cells in the attached population may have indicated radiosensitisation which would not have been detected by analysis of floating cells.
We examined those cells lines in which checkpoint abrogation was not associated with radiosensitisation, as assessed by increase in apoptosis, for the presence of giant cells in the attached population.
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Apoptosis assays were also performed on the Cell Viability Analyzer by quantifying the fraction of trypan blue-positive cells in the combined floating and attached populations with each assay being performed in triplicate.
However, there was no increase in apoptosis in attached cells (for example in S/RG/C2 the level of apoptosis in the attached cell population was consistently less than 1% in control and treated cultures).
We cannot expect a protracted guerilla resistance from a nation which has neither an inaccessible territory, nor a regular army, nor an attached peasant population.But if the Confederacy cannot long defend itself, if the civil war must soon come to an end, what will be its effect on us?
Further data are required on the distribution of ciliate protozoa in the rumen of healthy animals to improve model development, but the model described herein does indicate that the attached protozoal population is a significant component of the total rumen protozoal community.
There was no evidence, however, for an increase in apoptosis or giant cell formation in the attached cell population in those cultures treated with combined IR and UCN-01 compared to either treatment alone.
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