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Roche GS FLX Titanium sequencing produced short reads of 300 400 bp in length, which were then sorted into individual species based on the attached index tag sequences.
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The adapted amplicons were then modified by attaching indices and Illumina sequencing adapters using the Nextera XT Index Kit by PCR as described in the manufacturer's protocol, enabling simultaneous sequencing of multiple samples, i.e. multiplexing.
The Nextera kit is able to construct libraries of broad sizes (500 to 2,000 bp) by adding a gel purification step, and is able to attach indexes for up to 96 samples as described in the Methods.
Staying on the subject of volatility, the fear index attached to the Nasdaq 100 Index (NDX), the VXN, is showing that the NDX may outpace the broader market.
You should attach an index card with the preparation instructions written on it to the jar of pancake mix.
However, it should be noted here that as DRGs represent average price and are of varying sizes, so are the values attached to the index vignette, as that of the DRG to which the index vignette is assigned, retains that variability.
It works in much the same way, though; sensors are wrapped around the test subject's fingers, a clip is attached to their index finger, and a heart rate monitor is strapped to their chest.
Bilateral postural tremor was recorded using accelerometers attached to each index finger.
The GSR signals were measured using two electrodes which were attached to the index and middle fingers of the subject's right hand with Ag/AgCl gel materials.
Prior to the study, a palpometer, with which a pressure sensitive plastic film attached to the index finger records the pressure exerted, was used to train the observers.
The grasping angle was defined as the angle between the line defined by the DriveBay probes attached to the index finger and thumb, and an imaginary line along the vertical edge of the screen.
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CEO of Professional Science Editing for Scientists @ prosciediting.com