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Recordings were performed in cell attached configuration and whole-cell mode using an EPC-9 amplifier and Pulse/Pulsefit software (Heka Elektronik, Lambrecht, Germany).
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In the experimental series reported here, over 500 attempts were made to obtain a GigaOhm seal in cell attach configuration, among which 284 were successful.
Mhasin et al. attributed the tunable fluorescence of GQDs to their size, edge configuration, shape, attached chemical functionalities, heteroatom doping, and defects [22].
The controller consists of a DC motor, itself a part of a simple regulated feedback system, with a rigid beam/tip mass configuration attached to the motor shaft.
The US was an uncomfortable electric shock, delivered via a pin-cathode/ring-anode configuration attached to the dominant forearm.
We also examined spontaneous fast current transients (CTs) recorded from these pyramidal neurons under cell-attached configuration.
In the loose cell-attached configuration, it is possible both to stimulate individual cells and to record their action potentials, but most commercially-available equipment will not enable combining stimulation and recording.
The cell-attached configuration of the patch-clamp technique respects the integrity of the intracellular milieu.
In the cell-attached configuration the imposed membrane potential (Vm) is referred to as -Vp.
This is equivalent to the standard cell-attached configuration for the INM, but outside-out for the ONM.
Currents were recorded using an EPC9 patch-clamp amplifier (HEKA Elektronik, Lambrecht, Germany) in the "nucleus-attached" configuration.
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