Spring buds of P. australis with rhizome attached were collected on 8 September 2011 from Cherry Lake.
Hypothalamic blocks with the PT and pituitary attached were collected for immunohistochemistry, EM, and transcriptomics.
Small pieces of uterus with placenta attached were collected from days 19 21, 22 24, and 25 26 of pregnancy and fixed in 4% PFA before paraffin embedding.
All cells from the wells, floating and attached, were collected, washed and divided into three portions: one portion was fixed with 4% paraformaldehyde in 0.1 M phosphate buffer, washed and permeabilised with Triton X-100 for 5 min followed by labelling with rabbit α-LC3B (Novus-1384, Novus Biologicals, Littleton, CO, USA) for 2 h at room temperature.
Before two external bags were attached to collect his waste, Mr. Garst's urine leaked into his rectum because a fistula had developed.
There is no benefit attached to collecting all marks found at a crime scene and submitting them for further analysis regardless of their forensic quality.
Floating and attached cells were collected and combined and flow cytometry performed.
Coronal cryosections (5 µm) were serially collected, attached to glass slides, counterstained with Meyer's hematoxylin and Congo Red.
Nuclei of cells were stained with 4', 6-diamidino-2-phenylindole (DAPI) and images of the attached cells were collected using microscope.
For analysis of cell cycle distribution, cells (9×105) were plated into 100-mm tissue culture dishes and at approximately 30% confluence treated with 5 µM 4-oxo-4-HPR and/or 100 µM vitamin C. At different time points after the treatment, floating and attached cells were collected and washed twice with PBS, fixed in ice cold 70% ethanol and stored at -20°C until use.
