Sentence examples for attach to the plate from inspiring English sources
The KB cells with the concentration of 3 × 104 cells/ml were equally seeded in each well of a 96-well flat-bottom tissue culture plate and allowed to attach to the plate overnight.
Cells (1×104 cells/well) were seeded in a 96-well plate and incubated for 24 h to allow them to attach to the plate.
Despite this, some EBs still tended to attach to the plate, so every day the attached EBs were detached by gentle scraping with a sterile cell scraper.
The biopsy was immediately minced using sterilized forceps in a 60 mm dish containing 10% DMEM F12 media with 2.5 mg/l antibiotic/antimycotic (Invitrogen Life Sciences, Carlsbad, CA) and incubated for 10 14 days until fibroblasts started to attach to the plate.
These cells were allowed to attach to the plate surface for 24 h.
Cells were seeded into 96-well plates at 104 viable cells per well and left to attach to the plate for 24 hours.
Primers used for qPCR are included in Supplementary Data 2. The log-phase cells were plated in six-well plates overnight allowing cells to attach to the plates.
After brief washing with MDM, the neural crest tissue explants were placed on fibronectin-coated plates in MDM and incubated for 30 min to allow the explants to attach to the plates.
Cells were allowed to attach to the plates for 60 minutes at 37°C.
The cells were allowed to settle and attach to the plates for 6 to 12 hours, and formalin-fixed erythrocytes were then added and allowed to settle for 10 to 15 minutes.
