However, the presence of native paramagnetic centers and the ability to covalently attach site-directed spin labels in RCs makes them ideally suited for the application of SDSL EPR spectroscopy.
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The bacteria express ADHII with a linker 1 attached site specifically to their surface.
A quinone, an electron transfer mediator, was covalently attached site specifically to the displayed ADHII.
This novel approach was created to determine: (1) What will happen if a quinone is covalently attached site specifically into a predetermined site, in a hydrogenase that uses NAD+ as its cofactor.
Chlorin e6 c e6) was attached site specifically to the F ab' 2 fragment of the murine monoclonal antibody OC125, directed against human ovarian cancer cells, via poly-1-lysine linkers carrying cationic or anionic charges.
For many applications, the probe needs to be attached site-specifically and rigidly to the protein and the coordination of the lanthanoid by the tag must result in a unique anisotropy of the magnetic susceptibility.
Using solution X-ray scattering we determine the scattering interference between two gold nanocrystal probes attached site-specifically to a macromolecule of interest.
The main conclusion of general interest is that the tertiary structure, backbone dynamics, and conformational equilibria of T4L derivatives attached site-selectively to a Sepharose matrix are unperturbed relative to the protein in solution for the sites investigated.
The protocols described resulted in functional antibodies and were used to generate immunoconjugates containing two biotin moieties per protein molecule, attached site-specifically to the C-termini of the heavy chains.
This suggests that all plasmatocytes use the same form of attachment site to attach to both lateral and dorsal patches of the sessile compartment.
