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This first step allowed the hESC colonies to attach properly, and as expected cells continued to express high levels of Oct4 but not Brachyury or Sox17 (data not shown).
Having identified rampant spindle-pole amplification upon Red-Br-nos treatment, we wondered if MTs emanating from poles were able to attach properly to kinetochores.
These wings experience mechanical conditions similar to those in dumpy ov1 wings in that they undergo hinge contraction but cannot attach properly to the distal cuticle.
Nanofiber coatings 6 (n = 3) and 2 + HyA (n = 3, Figure 5 e)) did not support the growth of NRCs as well as the control surface and the cells did not attach properly on these coatings.
Failure to attach properly to the plasma membrane may be related to defects of ciliogenesis and it is possible that the same defect(s) that forms the basis for aberrant ciliogenesis also impacts centriole structure.
The alternative option suggested by the reviewers involving the use of immunocytochemistry would be technically challenging given that freshly sorted and plated TGFP+ cells require a few hours to attach properly thus precluding antibody staining at the very start of the experiment.
Similar(52)
Mercedes sent Nico Rosberg out without his right rear attached properly.
This sound department employee makes sure that all the cables are attached properly to the microphones and that they're working properly.
There was a slight malfunction of the wings — they weren't attaching properly to the Angel's back — so an alternate pair was brought in.
Michael Wolf, who runs the Greco Brush Company, a supplier to professional house painters, told me that his customers need to know before each job that every single bristle on every single brush will be attached properly.
When the volume of porogen decreases than 10 mL, the polymer pores shrined and the analyte was unable to get attached properly.
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CEO of Professional Science Editing for Scientists @ prosciediting.com