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5×10 MSC were seeded per well (6-well plate) and given time to attach for one day.
Bacterial cultures at 0.05 MacFarland were then used to inoculate the output wells; bacteria were pushed through the flow cell from the output well up to the horizontal microfluidic channel by reversing the flow and were allowed to attach for one hour followed by 16 h of incubation at 37 °C in BHI or 0.5xBHI with 0.1 % glucose at a flow rate of 0.5 dyne/cm.
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We developed a protocol to allow the collagen gel to polymerize into a gel around the cells for an hour, then render the gels floating or attached for one hour.
The Dragon capsule will attempt to connect with the ISS on June 4, and it'll remain attached for about one month before it returns to earth with a planned splashdown in the Pacific Ocean.
"No Strings Attached" is, for one thing, the first boy-band album with a song about video cybersex, "Digital Get Down," a clear indicator of post-pubescent consciousness.
For adhesion based tumor enrichment experiments the cells were allowed to attach at 37°C for one hour then all none adherent cells were transferred to a new flask and allowed to attach for 24 h.
The reviewers have discussed the reviews (which are attached for information) with one another, and the Reviewing Editor has drafted this decision to help you prepare a revised submission.
Although old controversies fade away and reemerge, they also reemerge profoundly transformed, and it is likely this transformation to which one can attach one's hope for progress in science in spite of recurring themes appearing.
The meals are packaged in a plastic clamshell that opens into attached bowls -- one for food, one for water.
X-ray photoelectron spectroscopy demonstrates that one C60 molecule is covalently attached for every ∼130 carbon atoms of the graphene C60.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com