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Tunicate larvae either seek out a place where they can attach and metamorphose into an adult or develop into adults that float in the open water.
The ciliated larvae, spherical and often about 1/4 millimetre in diameter, are liberated when fully developed and may swim first toward the light and thus away from the parent colony; later, however, the larvae avoid light as they seek a place in which to attach and metamorphose.
The nauplius larvae transformed into cyprids (ready to attach and metamorphose) after 3.5 days.
Cyprid larvae from each batch were settled on acrylic plastic (Plexiglas) panels and were transferred to the experimental system (see below) after eight days, leaving enough time for the larvae to attach and metamorphose before the start of the experiment.
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Control tadpoles are usually 100percentnt attached and metamorphosing 24 hr.
The effects of the test compounds against the larvae settlement were determined by examining the plates under a dissecting microscope to check for (1) settled larvae (attached and metamorphosed), (2) swimming larvae, as well as (3) any possible toxic effects of the treatments (dead and paralyzed).
In experiments with both TBP and CCA, only those larvae that had not been triggered to metamorphose after 6 h, such as in the 1∶1,000 dilution of synthetic TBP (Fig. 5C), attached and metamorphosed in response to CCA (Fig. 5D).
Planula-larvae sink to the seabed, settle and metamorphose into scyphistoma-polyps.
Inside they pupate and metamorphose into their adult winged form.
As the larvae mature and metamorphose, their limbs with digits become visible and the gills are resorbed.
Larvae were collected upon release from adults and metamorphosing individuals were obtained by allowing larvae to settle and metamorphose on a plastic petri dish as previously described [ 41].
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