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Sorted cells were plated onto laminin/polylysine-coated chamber slide dishes (Labtek) and allowed to attach and expand in SKP medium I.
In this work, we have a primary interest in the ability of cultured human meniscus cells to attach and expand within this carrier, and to produce collagens and proteoglycans between and around these 3D cultured cells.
Cells were seen to attach and expand in the serine-based gels despite the lack of encapsulated growth factors such as TGF-β and FGF-2, a finding that we consider to be significant.
We did not expect the cells to attach and expand well in 1, which also does not contain an adhesion motif and only differs chemically from 4 in the addition of six methyl groups in the hydrophilic face.
It is unclear why the SHEDs are able to attach and expand in the hydrogels of MDP 1 while they remain rounded in 4. The confocal data demonstrates that the chemistry of the hydrogel can have a significant effect on encapsulated SHED cells.
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When seeded on RGD-grafted CPC, NELL1-iPSC-MSCs attached and expanded similarly well to RFP-iPSC-MSCs.
When cultured on laminin-coated glass coverslips, the cancer cells attached and expanded in adherent monolayer on the substrate.
Therefore, we initially assessed the ability of hESCs to attach to and expand on three different microcarriers in conjunction with different dissociation regimes.
In the past year, a handful of papers have appeared reporting proof-of-concept studies demonstrating the ability of hESCs to attach to and expand on microcarriers.
Theories for the postnatal presence and then reduction of TNC within the articular cartilage include maintenance of the chondrocyte phenotype, capability of the cells to attach and then detach in an expanding matrix, and the utilization of signal transduction for mediating chondrocyte developmental fate [ 38].
The lumbar vertebrae are attached to the diaphragm, which main function is to contract and expand the lungs during inspiration.
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