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For the transiently transfected cells, they were placed in the atmospheric box at the various oxygen concentrations as indicated in the figures.
Cells were maintained in a stage-mounted atmospheric box (Pathology Devices, Westminster, MD, USA) at 37 °C, 5% CO2, and 75% humidity during the course of the experiments.
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When I initially meet Refn, he is being photographed in a darkly atmospheric boxing gym a few minutes' walk from the Observer offices.
We use an extended version of an atmospheric chemistry box model including detailed gas-phase chemistry, mixing layer height variation and secondary organic aerosol calculations based on observations for Berlin, Germany.
Since densities of CO2 and N2O are higher than air (δCO2 = 1.842, δN2O = 1.872, δ air = 1.205 at room temperature and atmospheric pressure), the box fills progressively until the gas escapes by overflow.
Then they slowly pumped air out of the box, simulating the atmospheric conditions at higher and higher altitudes.
For probe calibration and monitoring of oxygenation under graded hypoxia, cells stained with Ir1 (10 μ m, 16 h) were exposed to different levels of atmospheric O2 in a glove box (Coy Scientific), with 60 min pre-incubation and 30 60 min measurement at each O2 concentration in the presence of antimycin A (10 μ m).
Ermita 104, +51 1477 1120 Impressively atmospheric bar of low lights and heavy decoration, full of statuettes and images of angels, colonial art, incense - even a red telephone box, which you assume is from Britain until you read "telefono" at the top.
This would decrease the oxygen concentration within the boxes back to atmospheric conditions.
The pressure of the experimental box was established at atmospheric pressure within a minute.
Animals were collected with the telemanipulated arm of Nautile in insulated boxes 4-5°C 4-5°Ccoverecoveredmospheric pressure but dissected atfew minutes atmospheric recovered onboard in RNALater stabilization and storage solution.
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