Exact(4)
Several measurable changes were recorded in the mode of decomposition of all these complexes when the atmosphere was changed from nitrogen (inert) to air (reactive).
The nitriding atmosphere was changed from pure nitrogen to the richest hydrogen with substrate temperatures ranging from 600 to 900 °C.
Then, the atmosphere was changed to nitrogen, 10 mL of dry toluene and 0.307 mmol of aminopropyltrimethoxysilane (APTMS; 55.04 mg) were added, and the solution was refluxed for 24 h under nitrogen.
The atmosphere was changed to a 5% O2 (hypoxia) or a 0.1% O2 (anoxia) atmosphere by flushing vials with premixed gas O2/N2 mixtures (Linde Gas) [ 8].
Similar(56)
Cells were incubated at 37°C in a 5% CO2 humidified atmosphere; medium was changed in both chambers 24 h post-seeding and then every 2-3 days.
Cells were maintained at 37°C in a 95%air-5air-5%bon dioxide (CO2) humidified atmosphere, and medium was changed every 2 3 d.
Cells were cultured in a humidified incubator at 37°C with a 5% CO2 atmosphere and the medium was changed every second day.
The cells were incubated in a cell culture incubator (Heraeus, BB15, Germany) at 37°C, 5% CO2 in a humidified atmosphere and the medium was changed every 48 h.
For the measurements at different oxygen partial pressures, the atmosphere in the quartz reactor was changed to different pO2 values using various O2 Ar and air Ar gas mixtures, and monitoring the pO2 of the outlet gas with a zirconia oxygen sensor.
All cultures were incubated at 37°C in a 5% CO2 enriched humid atmosphere and the proliferation medium was changed every other day.
After 5 h incubation in a humidified 5% CO2 atmosphere at 37°C, Opti-MEM was changed to growth medium and the cells were further incubated for 18 or 24 h.
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