Sentence examples for atmosphere cell from inspiring English sources

Only the sample has to be placed inside a controlled atmosphere cell.

The experiments were performed in a controlled argon atmosphere cell with safety features.

After 24 hrs incubation at 37°C in a 5% CO2 atmosphere, cell membranes were removed and cells were fixed for 30 min in 100% ice cold methanol.

After 1 hour incubation at 37°C in a 5% CO2 atmosphere cells were washed three times in PBS and lysed by addition of 1 mL 0.1% Triton X-100 for five minutes.

Multiwell plates were centrifuged at 400 g for 10 min After 1 h of incubation at 37°C in a 5% CO2 atmosphere, cells were gently washed with PBS (pH 7.4) and then incubated for 1 h in medium supplemented with 100 µg/ml of Str and 50 µg/ml of Gm to kill remaining extracellular bacteria.

In order to ensure close contact between cells and bacteria, multiwell plates were centrifuged at 400 g for 10 min After 30 min of incubation at 37°C in a 5% CO2 atmosphere, cells were gently washed with phosphate buffered saline (PBS, pH 7.4) and then incubated for 1 h in medium supplemented with Str (100 µg/ml) and Gm (50 µg/ml) to kill remaining extracellular bacteria.

After incubation for 12 h at 37°C in 5% CO2 atmosphere, cells were either maintained in glucose medium or switched to galactose medium for 12 h.

Using typical bicarbonate buffer and a 5%CO2/95%% air atmosphere, cells are exposed to much higher levels oxygen compared with what they would be exposed to in vivo.

Briefly, after overnight incubation at 37 °C in 5% humidified atmosphere, cells were harvested, lysed, washed with PBS and incubated with Annexin V-FITC (Pharmingen, San Diego, CA) and propidium iodide (PI) according to the manufacturer's manual.

After additional 4 h at 37°C in a 5% CO2 atmosphere, cells were fixed for 15 min at RT by adding 30 μl of 8% PFA in PBS to each well using a multidrop 384 device (Thermo Electron Corporation).

After 24 h incubation at 37°C in a 5% CO2 atmosphere, cells that migrated to the lower surface of filters were detected with traditional staining with hematoxylin and eosin.